Living Networks is pioneering a novel approach towards vascular tissue engineering based on highly precise manipulation of cell microcarriers and their assembly into microarrays.
Each microcarrier gives rise to an independent, vascularized microenvironment, representing the human vasculature at the microscale.
Medium
Hydrogel & Cells
Magnet
Our advanced image analysis software enables rapid, multi-parametric phenotypic assessment of each microtissue to deliver streamlined workflows.
Compatible with standard multi-well formats, VTµA platform integrates seamlessly with bio-analytical assays across proteomics, genomics, and histology, advancing vascular tissue research and therapeutic development.
Vascularized Tissue micro-Arrays (VTµA)
For High-Throughput Phenotypical Screening.
The smallest vascularized human tissue model ever made.
Automation of
morphometric readouts.
The unique microcarrier approach as well
as our proprietary software allow for automation of the morphometric measurements, eliminateing bias and warraningt high precision.
Physiological relevance and flexibility.
Each microtissue consists of functional human microvessels, embedded in 3D extracellular matrix and tissue cells of choice; we offer both healthy and diseased tissue models.
Ultra-high throughput.
Our 3D angiogenesis assays are available in standard multi-well formats at ultra-high throughput with up to N=1944 microtissues per plate.
Cost-efficiency.
Ultra-low consumption of cells and reagents (≈0.2 million endothelial cells per 48-well plate), facilitating assays even with scarce biological material, e.g., from patient biopsies or hiPSC-derived sources.
Publications
Long-term day-by-day tracking of microvascular networks sprouting in fibrin gels: From detailed morphological analyses to general growth rules
Katarzyna O. Rojek, Antoni Wrzos, Stanisław Żukowski, Michał Bogdan, Maciej Lisicki, Piotr Szymczak, Jan Guzowski
Engineering of interconnected microcapillary networks at the mesoscale via magnetic assembly of endothelial-cell ‘seeds’
Katarzyna O. Rojek, Antoni Wrzos, Fabio Maiullari, Konrad Giżyński, Maria Grazia Ceraolo, Claudia Bearzi, Roberto Rizzi, Piotr Szymczak, Jan Guzowski